Journal: World Journal of Gastroenterology
Article Title: Mesalazine alleviated the symptoms of spontaneous colitis in interleukin-10 knockout mice by regulating the STAT3/NF-κB signaling pathway
doi: 10.3748/wjg.v31.i7.96459
Figure Lengend Snippet: Effect of mesalazine on alleviating endoplasmic reticulum stress in interleukin-10 -/- mouse colonic epithelial cells. A: Transmission electron microscope photograph of mouse colonic epithelial cells (magnification × 8200); B: Glucose-regulated protein 78 protein level; C: Representative images of glucose-regulated protein 78 immunofluorescence (green) and nuclei (blue) in colonic tissue; D: Representative images of C/EBP homologous protein immunofluorescence (green) and nuclei (blue) in colonic tissue. Compared with the interleukin-10 -/- group, a P < 0.05, b P < 0.01. WT: Wild type; IL: Interleukin; ASA: Amino salicylic acid; GRP78: Glucose-regulated protein 78; DAPI: 4’,6-diamidino-2-phenylindole; CHOP: C/EBP homologous protein.
Article Snippet: They were incubated overnight at 4 °C with rabbit anti-GRP78 polyclonal antibodies (1:100, 11587-1-AP, Proteintech) and rabbit anti-CHOP antibodies (1:100, 5554S, Cell Signaling Technology).
Techniques: Transmission Assay, Microscopy, Immunofluorescence